Course: Methods of Molecular Biology

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Course title Methods of Molecular Biology
Course code 3160/FAMG1
Organizational form of instruction Lecture
Level of course Master
Year of study not specified
Semester Winter
Number of ECTS credits 2
Language of instruction English
Status of course Compulsory-optional
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
  • Hošek Jan, RNDr. Ph.D.
Course content
1) Isolation and characterisation of nucleic acids and proteins 2) Centrifugation 3) Electrophoresis - agarose, polyacrylamide, pulse-field, capillary 4) Modifying enzymes 5) Restriction endonucleases 6) Polymerase chain reaction including real-time PCR 7) Real-time PCR 8) Southern a northern blotting, hybridisation, DNA and RNA chips 9) Sequencing of DNA and proteins 10) ELISA, western blotting 11) Basic calculation in molecular biology

Learning activities and teaching methods
  • unspecified - 5 hours per semester
  • unspecified - 12 hours per semester
  • unspecified - 12 hours per semester
Learning outcomes

Fundamental knowledge of principles of methods used in molecula biology, which create basics for following lectures Drug biotechnology, Practices of drug biotechnology, Pharmacogemonics, Practices of pharmacogenomics.
3120/F1ME1 Molecular biology

Assessment methods and criteria
At least of 50% points from final test.
Recommended literature
  • Barker K. At the Bench. A laboratory navigator.. Cold Spring Harbor Laboratory Press, USA, 2005.
  • Bustin SA. A-Z of quantitative PCR. International University Line, USA, 2004.
  • Bustin SA. A-Z of quantitative PCR. International University Line, USA, 2004.
  • Sambrook a Russell. Molecular cloning, A laboratory manual. the 3rd edition. Cold Spring Harbor Laboratory Press, New York, USA, 2001.
  • Šmarda J, et al. Metody molekulární biologie. Masarykova Universita Brno, 2005.

Study plans that include the course
Faculty Study plan (Version) Branch of study Category Recommended year of study Recommended semester
Faculty of Pharmacy - (12) Pharmaceutical sciences 3 Winter