Course: Biotechnology of Drugs

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Course title Biotechnology of Drugs
Course code 3160/FABL1
Organizational form of instruction Lecture
Level of course Master
Year of study not specified
Semester Summer
Number of ECTS credits 3
Language of instruction English
Status of course Compulsory-optional
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
  • Hošek Jan, RNDr. Ph.D.
  • Kizek René, Prof. Ing. Ph.D.
  • Brázdová Marie, Mgr. Ph.D.
Course content
1) Introduction to pharmaceutical biotechnology, definition, milestones 2) The cell as a tool of biotechnology, function of endoplasmic reticulum, Golgi komplex, posttranslational processes, chaperones 3) The basics of gene engineering I - the principles, how to prepare recombinant gene, vectors 4) The basics of gene engineering II - preparation of genes for cloning, host cells, transformation, selection and transformants identification 5) Expression of recombinant proteins in prokaryotic cells and yeasts 6) Expression of recombinant proteins in eukaryotic cells - insect cells and baculoviruses, mammalian cells and adenoviruses 7) The classical biotechnological processes in pharmacology 8) The process of biotechnology - definition, the phases of biotechnological process, basic materials, fermentors and bioreactors, methods of biotechnological products purification 9) Examples of classical and recombinant biotechnology products in pharmacy; cytokines, hormones, enzymes, antibodies and their derivates 10) Gene engineering of plants - structure of plant genome, vectors, expression cassettes 11) Plant biotechnology - methods of transformation and transgene identification, molecular pharming 12) Application of gene engineering and plant biotechnology in pharmacy, medicine, and food industry; rules for genetically modified organisms manipulation 13) Gene and cell therapy, tissue engineering, genomics and proteomics in pharmacy 14) New trends in biopharmaceutical products development

Learning activities and teaching methods
  • unspecified - 12 hours per semester
  • unspecified - 24 hours per semester
  • unspecified - 36 hours per semester
Learning outcomes
The course is demonstration of Pharmaceutical Biotechnology - scientific discipline focusing on all technologies required for the production, manufacturing and registration of biotechnological drugs. Students will be introduced to processes which are used by organisms or their parts to production or modification of drugs. The methods used for breeding of animals, plants and microorganisms usable in medicine and pharmacy will be discussed. The main focus is done for recombinant techniques, gene and protein engineering and gene therapy. Products of industrial biotechnology as antibiotics, vitamins, organic acids, amino acids and alkaloids, will be presented.
Basic knowledge from cytology, gene engineering and industrial microbiology usable in recombinant drugs design, development and production. View to biotechnological production of antibiotics, vitamins, organic acids, amino acids and alkaloids.
3140/FAOB1 Biology 3120/FAME1 Molecular biology 3120/FAMG1 Methods of molecular biology

Assessment methods and criteria
Credits from practical course Test: at least 50 % of credits Oral examination
Recommended literature
  • Alberts et al. (1998). Molecular Biology of the Cell, 5th edition, GS Garland Science.
  • Brown, T.A. (2010). Gene cloning and DNA analysis, an introduction. 6th edition, Wiley-Blackwell.
  • HO, R. J. Y. ; GIBALDI, M. Biotechnology and Biopharmaceuticals. Transforming proteins and genes into drugs. John Willey and Sons, Hobokey, New Jersey, U.S.A., 2003.
  • KAYSER, O.; MÜLLER, R.H. Pharmaceutical Biotechnology. Wiley-VCH Verlag GmbH and Co. KGaA, Weinheim, Germany., 2004.
  • SLATER, A.; SCOTT, N.; FOWLER, M. Plant biotechnology. The genetic manipulation of plants.. Oxford University Press. Oxford, New York, U.S.A., 2003.
  • Walsh, G. (2007). Pharmaceutical Biotechnology, John Wiley and sons.

Study plans that include the course
Faculty Study plan (Version) Branch of study Category Recommended year of study Recommended semester
Faculty of Pharmacy - (12) Pharmaceutical sciences 3 Summer