Course: Biotechnology of Drugs, practical classes

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Course title Biotechnology of Drugs, practical classes
Course code 3160/F1BL2
Organizational form of instruction Lesson
Level of course Master
Year of study not specified
Semester Summer
Number of ECTS credits 3
Language of instruction Czech
Status of course Compulsory-optional
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
  • Hošek Jan, RNDr. Ph.D.
  • Nejezchlebová Marcela, Ing.
  • Treml Jakub, PharmDr. Ph.D.
Course content
1) Isolation of plant chromosomal DNA 2) Amplification of selected plant gene by polymerase chain reaction 3) Purification of PCR products 4) Isolation of plasmid DNA 5) Nucleic acids analysis by agarose gel electrophoresis, spectrophotometry 6) Digestion of vector DNA by restriction endonucleases 7) Ligation of PCR product into a vector 8) Transformation of bacterial cells Escherichia coli 9) PAGE

Learning activities and teaching methods
  • unspecified - 5 hours per semester
  • unspecified - 30 hours per semester
Learning outcomes
This subject is mainly focused on cytology, molecular biology and microbiology principles, which are used in recombinant therapeutics preparation. Students will be familiarized with recombinant techniques, gene and protein engineering, and gene therapy as well. Attention will be also paid to industrial biotechnology and production of significant products, such as antibiotics, vitamins, organic acids, amino-acids and alkaloids.
Basic knowledge in methods of pharmaceutical biotechnology

Assessment methods and criteria
100% attendance at compulsory exercises laboratory protocols final test
Recommended literature
  • Bartoš, M,; Forejtníková, H.; Bartošová, L. Biotechnologie pro farmaceuty (Návody k praktickým cvičením). VFU Brno, 2006.
  • Sambrook, J.; Russell, D. W. Molecular cloning, A laboratory manual. Cold Spring Harbor Laboratory Press, New York, USA, 2001.
  • Šmarda, J, et al. Metody molekulární biologie. Masarykova univerzita v Brně, 2005.

Study plans that include the course
Faculty Study plan (Version) Branch of study Category Recommended year of study Recommended semester
Faculty of Pharmacy Pharmacy (1) Pharmaceutical sciences 3 Summer