Course: Biotechnology of Drugs

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Course title Biotechnology of Drugs
Course code 3160/F1BL1
Organizational form of instruction Lecture
Level of course Master
Year of study not specified
Semester Summer
Number of ECTS credits 3
Language of instruction Czech
Status of course Compulsory-optional
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
  • Hošek Jan, RNDr. Ph.D.
  • Kizek René, Prof. Ing. Ph.D.
  • Brázdová Marie, Mgr. Ph.D.
Course content
1) Introduction to pharmaceutical biotechnology, definition, milestones 2) The cell as a tool of biotechnology, function of endoplasmic reticulum, Golgi komplex, posttranslational processes, chaperones 3) The basics of gene engineering I - the principles, how to prepare recombinant gene, vectors 4) The basics of gene engineering II - preparation of genes for cloning, host cells, transformation, selection and transformants identification 5) Expression of recombinant proteins in prokaryotic cells and yeasts 6) Expression of recombinant proteins in eukaryotic cells - insect cells and baculoviruses, mammalian cells and adenoviruses 7) The classical biotechnological processes in pharmacology 8) The process of biotechnology - definition, the phases of biotechnological process, basic materials, fermentors and bioreactors, methods of biotechnological products purification 9) Examples of classical and recombinant biotechnology products in pharmacy; cytokines, hormones, enzymes, antibodies and their derivates 10) Gene engineering of plants - structure of plant genome, vectors, expression cassettes 11) Plant biotechnology - methods of transformation and transgene identification, molecular pharming 12) Application of gene engineering and plant biotechnology in pharmacy, medicine, and food industry; rules for genetically modified organisms manipulation 13) Gene and cell therapy, tissue engineering, genomics and proteomics in pharmacy 14) New trends in biopharmaceutical products development

Learning activities and teaching methods
  • unspecified - 12 hours per semester
  • unspecified - 24 hours per semester
  • unspecified - 36 hours per semester
Learning outcomes
This subject is mainly focused on cytology, molecular biology and microbiology principles, which are used in recombinant therapeutics preparation. Students will be familiarized with recombinant techniques, gene and protein engineering, and gene therapy as well. Attention will be also paid to industrial biotechnology and production of significant products, such as antibiotics, vitamins, organic acids, amino-acids and alkaloids.
Basic knowledge from cytology, genetic engineering, and industrial microbiology which are used in preparation of recombinant pharmaceuticals. Overview of biotechnology production of antibiotics, vitamins, organic acids, amino acids and alkaloids.

Assessment methods and criteria
the exem consists of two parts: written test (student has tu obtain at least 50% of of credits) and oral examination.
Recommended literature
  • Alberts et al. Základy buněčné biologie, Úvod do molekulární biologie buňky. Espero Publishing, 1998.
  • Brawn. Klonování genů a analýza DNA. Univerzita Palackého v Olomouci,, 2007.
  • HO, R. J. Y. ; GIBALDI, M. Biotechnology and Biopharmaceuticals. Transforming proteins and genes into drugs. John Willey and Sons, Hobokey, New Jersey, U.S.A., 2003.
  • KAYSER, O.; MÜLLER, R.H. Pharmaceutical Biotechnology. Wiley-VCH Verlag GmbH and Co. KGaA, Weinheim, Germany., 2004.
  • Ondřej, M.; Drobník, J. Transgenoze rostlin. Academia Praha, 2002.
  • Ruml, al. Genové inženýrství. VŠCHT Praha, 2002.
  • SLATER, A.; SCOTT, N.; FOWLER, M. Plant biotechnology. The genetic manipulation of plants.. Oxford University Press. Oxford, New York, U.S.A., 2003.
  • Šmarda, J, et al. Metody molekulární biologie. Masarykova univerzita v Brně, 2005.
  • Vodrážka, Z. Biotechnologie. VŠCHT Praha, 1991.

Study plans that include the course
Faculty Study plan (Version) Branch of study Category Recommended year of study Recommended semester
Faculty of Pharmacy Pharmacy (1) Pharmaceutical sciences 3 Summer