Course: Microbiological Laboratory Methods

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Course title Microbiological Laboratory Methods
Course code 2340/H2MLM
Organizational form of instruction Lecture
Level of course Master
Year of study 2
Semester Winter
Number of ECTS credits 3
Language of instruction Czech
Status of course Compulsory-optional
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
  • Karpíšková Renata, Doc. MVDr. Ph.D.
  • Dušková Marta, Mgr. Ph.D.
  • Necidová Lenka, MVDr. Ph.D.
  • Bursová Šárka, MVDr. Ph.D.
Course content
Lectures 1. 4 hours Automated system used in detection and identification of microorganisms: TEMPO system, VITEK system, Biolog system, biosensors, mass spectromectry MALDI-TOF. Modern methods used in food microbiology. Immunological techniques and their use in food microbiology: agglutination techniques, ELISA, ELFA, LFIA. Indirect methods: electric methods, radiometry, LAL test, ATP-bioluminescence method. 2. 4 hours Molecular-biological methods used in food microbiology. DNA isolation, polymerase chain reaction, real-time PCR, hybridisation techniques, microarrays. 3. 4 hours Rezistance to antimicrobial agents importance, methods of detection. The disk diffusion method, agar dilution method, microdilution method, E-test, detection of resistance genes. Assignment case studies. 4. 4 hours Bacterial toxins in food importance, methods of detection. Methods for detection of staphylococcal enterotoxin, methods for detection of toxins produced by Bacillus cereus, determination of botulotoxins. 5. 4 hours Foodborne diseases methods used in epidemiology. Serotyping, phagotyping, PFGE, PCR with restriction digestion, VTNR (MLVA). 6. 4 hours Foodborne diseases - a case study

Learning activities and teaching methods
Learning outcomes

Assessment methods and criteria
Conditions for obtaining credit: - participation in lectures - preparing and defending a case study - exam: written test; passing score: 75 %
Recommended literature
  • Bursová, Š a kol. Mikrobiologické laboratorní metody. VFU Brno, 2014.
  • Dijkshoorn L. et al. New Approaches for the Generation and Analysis of Microbial Typing Data. 1st ed. Amsterodam: Elsevier, 2001..
  • Lewis, K. et al. Bacterial Resistance to Antimicrobials. 1st ed. NewYork: Marcel Dekker, Inc., 2002..
  • McMeekin, TA. Detecting pathogens in food. Cambridge: Woodhead Publishing Limited, 2003.
  • O'Connor, L. Diagnostic BacteriologyProtocols. Totowa: Humana Press, 2010.
  • Rosypal, S. a kol. Terminologie molekulární biologie. 1. vyd. Brno: Rosypal, 2001..
  • Šmarda, J, et al. Metody molekulární biologie. Masarykova univerzita v Brně, 2005.
  • Woodford, N. a Johnson, AP. Molecular Bacteriology. Protocols and Clinical Applications. 1st ed. Totowa: Humana Press, 1998..

Study plans that include the course
Faculty Study plan (Version) Branch of study Category Recommended year of study Recommended semester
Faculty of Veterinary Hygiene and Ecology Food Safety and Quality (1) Veterinary medicine and veterinary prevention 2 Winter