Course: Biochemistry I

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Course title Biochemistry I
Course code 2390/HABC1
Organizational form of instruction Lecture + Lesson
Level of course Master
Year of study not specified
Semester Summer
Number of ECTS credits 4
Language of instruction English
Status of course Compulsory
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Hostovský Martin, MVDr. Ph.D.
  • Kopřiva Vladimír, MVDr. Ph.D.
  • Nekvapil Tomáš, MVDr. Ph.D.
Course content
BIOCHEMISTRY LECTURES: 1 Introduction to Biochemistry. Saccharide metabolism I. Glycolytic pathway. Aerobic decarboxylation of pyruvate 2 Saccharide metabolism II. Gluconeogenesis. Cori-Alanine cycle. Glycogen biosynthesis and breakdown. 3 Saccharide metabolism III. Fructose and galactose metabolism. Pentose phosphate pathway. Glucuronate pathway. 4 Citric acid cycle, energetic yield. Glyoxylate cycle. 5 Lipid metabolism I. Digestion and absorption of lipids. Biosynthesis and degradation of FFA. 6 Lipid metabolism II. Biosynthesis of cholesterol and its transformation to bile acids, steroid hormones, calciols. 7 Lipid metabolism III. Komplex lipids - phospholipids and glycolipids. Synthesis of eicosanoids. 8 Lipid metabolism IV. Transport forms of cholesterol lipoproteins, mixed micelle. 9 Lipid metabolism V. Metabolism of ketone bodies. Metabolism of adipose tissue. 10 Enzymes I. Characteristic features of biocatalysis, enzyme structure and function, nomenclature and classification of enzymes. Allosteric enzymes. 11 Enzymes II. Enzyme cofactors, review of structures and functions. Enzyme kinetics. Inhibition of enzymes. 12 Enzymes III. Diagnostic enzymes and their clinical importance. Isoenzymes. Metaloenzymes. Macroenzymes 13 Protein metabolism I. Metabolism of proteins. Protein and amino acid metabolism. The common reactions in amino acid degradation. 14 Protein metabolism II. Catabolism of proteins. Deamination of amino acids. Non-toxic transportation forms of ammonia. Glutamine cycle. Urea synthetic cycle. BIOCHEMISTRY PRACTICALS: W: Exercise No.: 1 Introduction to practicals in Biochemistry, rules, work safety, conditions for credit. 2 No.1-MINERALS I. Determination of magnesium ions in the blood serum. Determination of inorganic phosphate in the blood serum. 3 No.2-MINERALS II. Determination of the concentration of iron in the blood serum . Determination of calcium ions in the blood serum. 4 No.3-VITAMINS The separation of the vitamin mixture by gel permeation chromatography on Sephadex G-25. A rough estimate of ascorbic acid in urine by iodometric titration. 5 No.4-AMINO ACIDS Thin layer chromatography (TLC) the separation of amino acids. 6 Biological materials, results interpretation. Written test I. 7 No.5-SACCHARIDES Enzymatic determination of glucose in blood serum (page 62). Enzymatic determination of glucose in model samples (page 68). 8 No.6-LIPIDS Quantitative determination of total cholesterol in blood serum (77). Qualitative determination of lipoperoxidation in oils. 9 No.7-ENZYMES I. The substrate specifity of the enzymes (47). Determination of alkaline phosphatase (ALP) activity in the blood serum (41). 10 No.8-ENZYMES II. Determination of Asparate aminotransferase (AST/ASAT) activity in blood serum (91). Determination of Gamma-Glutamyl transferase (GMT/GGT) activity in blood serum (96). 11 Control sera for GLP (Good Laboratory Practice). Written test II. 12 GLP Determination of proteins in the blood serum and Lyonorm using Biuret reagent. 13 Substitutionary lesson. Consultations, checking protocols. Credit test. 14 Credit test repetition. Credit.

Learning activities and teaching methods
unspecified
Learning outcomes
Biochemistry in the summer semester introduces students to the metabolic conversion of the basic substances in the organism and food stuff (i. e. carbohydrates, lipids, amino acids) with the appropriate enzymes that catalyze this metabolic conversion. Individual metabolic pathways are located in the specific compartments of the cell, and they are important for the correct function of the cell/tissue for example by production of ATP or other products.
Knowledge gained from Biochemistry I. and especially their understanding is essential for the second semester of biochemistry, where the knowledge continues and expands for additional metabolite involving in more complicated metabolic pathways.
Prerequisites
The conditions for getting the credit from the subject Biochemistry are 100% attendance, preparedness for the practical lessons during the whole semester according to the requirements which are set by the head of discipline at the beginning of the semester. Passing all the tests and elaboration of all protocols with the signature of the assistant are also very important for the credit. In the case of inadequate preparation for the practical lesson, the student can be excluded from training. The compensation of the lesson must be done according to the assistant's instructions (i. e. substitution at the end of the semester or elaboration of the seminar work). When a student doesn't get a credit, he (she) must write a credit test that has only three attempts.

Assessment methods and criteria
unspecified
The conditions for getting the credit from the subject Biochemistry are 100% attendance, preparedness for the practical lessons during the whole semester according to the requirements which are set by the head of discipline at the beginning of the semester. Passing all the tests and elaboration of all protocols with the signature of the assistant are also very important for the credit. In the case of inadequate preparation for the practical lesson, the student can be excluded from training. The compensation of the lesson must be done according to the assistant's instructions (i. e. substitution at the end of the semester or elaboration of the seminar work). When a student doesn't get a credit, he (she) must write a credit test that has only three attempts.
Recommended literature
  • CHAMPE,P.C.;HARVEY,R.A. Lippincott´s Illustrated Reviews: Biochemistry.. Lippincott Wiliams & Wilkins, 1994. ISBN 0-397-51091-8.
  • J. Koolman, K. H. Röhm. Color Atlas of Biochemistry.
  • MURRAY,R.K.; GRANNER,D.K.;RODWELL,V.W. Harper´s Illustrated Biochemistry.. Appleton & Lange, 2006. ISBN 07-147885-X.


Study plans that include the course
Faculty Study plan (Version) Branch of study Category Recommended year of study Recommended semester
Faculty of Veterinary Hygiene and Ecology - (12) Veterinary medicine and veterinary prevention 1 Summer